| 中文名稱 | 線粒體脫偶連蛋白2抗體 |
| 別 名 | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_MOUSE. |
| 研究領域 | 細胞生物 免疫學 染色質和核信號 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Pig, Horse, Rabbit, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse UCP-2:201-309/309 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Subunit: Acts as a dimer forming a proton channel (By similarity). Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: P70406 Gene ID: 7351 Database links: Entrez Gene: 7351 Human Entrez Gene: 54315 Rat Omim: 601693 Human SwissProt: P55851 Human SwissProt: P56500 Rat Unigene: 80658 Human Unigene: 13333 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. UCP-2又稱解耦聯蛋白 2 (UCP2 )是 1997年發現的一種新的解偶聯蛋白 ,它是線粒體內膜載體家族的一員 。與其類似物UCP1相比 ,UCP2在棕色脂肪組織中含量較低 ,但在體內分布廣泛。解耦聯蛋白(UCP)具有使線粒體呼吸鏈解耦聯作用,解耦聯所釋放的能量以熱能的形式散發。在對UCP與產熱關系的研究中,發現UCP2具有調節機體能量代謝的功能,并且可能是肥胖與2型糖尿病相關聯的一個重要基因。 |
| 產品圖片 |  Sample:Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Du145 (Human) Cell Lysate at 30 ug Lane 4: A673 (Human) Cell Lysate at 30 ug Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Lane 1: Stomach (Rat) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: SiHa (Human) Cell Lysate at 30 ug Lane 4: DU145 (Human) Cell Lysate at 30 ug Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 ug Lane 6: Liver (Mouse) Lysate at 40 ug Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Stomach (Mouse) Lysate at 40 ug Primary: Anti- UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 32 kD  Sample:BV-2(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 32 kD  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug BV-2(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 33 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (bs-1926R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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