| 中文名稱 | 高遷移率族蛋白B1抗體 |
| 別 名 | Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_MOUSE. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse HMGB1:61-150/215 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. SWISS: P09429 Gene ID: 100862258 Database links:
Entrez Gene: 282691 Cow Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Entrez Gene: 25459 Rat Omim: 163905 Human SwissProt: P10103 Cow SwissProt: P09429 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 近來的研究表明稱之為高遷移率族蛋白B-1（HMG-B1）的核內(nèi)結(jié)構(gòu)蛋白在核外表達(dá)時(shí)是一種有效的早期炎癥介質(zhì)。 高遷移性Ｂ１組蛋白（ＨＭＧＢ１）： 是一種核結(jié)合蛋白，在ＤＮＡ重組、修復(fù)、復(fù)制和基因轉(zhuǎn)錄中起作用。ＨＭＧＢ１也是巨噬細(xì)胞分泌的一種介質(zhì)。 此外，高遷移性Ｂ１組蛋白亦被受刺激的巨噬細(xì)胞或單核細(xì)胞主動(dòng)分泌。在這一主動(dòng)分泌過程中，HMGB1首先經(jīng)乙酰化并由核內(nèi)轉(zhuǎn)移至溶酶體內(nèi)，繼而在ATP和溶血磷脂膽堿兩種分泌信號(hào)指導(dǎo)下轉(zhuǎn)移至細(xì)胞外。由壞死細(xì)胞被動(dòng)釋放的HMGB1和炎癥細(xì)胞主動(dòng)分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細(xì)胞因子參與信號(hào)傳導(dǎo)，因?yàn)樗瓤勺R(shí)別Toll 樣受體（TLR）家族的一些成員，又能與識(shí)別晚期糖基化終末產(chǎn)物受體（RAGE）相作用。 HMGB1能啟動(dòng)炎癥反應(yīng)，包括產(chǎn)生多種細(xì)胞因子、對(duì)某些干細(xì)胞產(chǎn)生趨化作用、誘導(dǎo)血管粘附分子、削弱腸上皮細(xì)胞的功能等。 |
| 產(chǎn)品圖片 |  Sample:MCF-7 Cell (human) Lysate at 40 ug Primary: Anti- HMGB1 (bs-20633R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25kD Observed band size: 27 kD  Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- HMGB1 Polyclonal Antibody (bs-20633R) at 1:300 for 3 hours at 4°C, followed by Rhodamine-conjugated secondary antibody for an additional hour. Blank control (blue line): MCF7 (fixed with 80% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-20633R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.  Blank control:HL-60.Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-20633R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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