| 中文名稱 | 磷酸化丙酮酸激酶M2抗體 |
| 別 名 | PKM2 (Phospho Tyr105); PKM2 (Phospho Y105); PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105:IL(p-Y)RP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Subunit: Monomer and homotetramer. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Post-translational modifications: ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Similarity: Belongs to the pyruvate kinase family. SWISS: P14618 Gene ID: 5315 Database links: Entrez Gene: 5315 Human Entrez Gene: 18746 Mouse Entrez Gene: 25630 Rat Omim: 179050 Human SwissProt: P14618 Human SwissProt: P52480 Mouse SwissProt: P11980 Rat Unigene: 534770 Human Unigene: 326167 Mouse Unigene: 405069 Mouse Unigene: 1556 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 丙酮酸激酶（PK）有L、R、M1、M2四種同功酶，均為四聚體。 L型主要分布于肝臟，R型存在于紅細(xì)胞，在結(jié)構(gòu)、免疫學(xué)和動力學(xué)上十分相似，由同一基因調(diào)控；M1存在于肌肉中，M2分布于除上述以外的其他組織（尤以腎臟最多）以及胎兒各組織。PK也是一種癌胚蛋白，在惡性腫瘤中，增高的都是M2型。 |
| 產(chǎn)品圖片 |  Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Phospho-PKM2 Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Rat Thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-NMDAR1(Ser890) Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugati Phospho-PKM2on to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-PKM2(Tyr105) Polyclonal Antibody, Unconjugated(bs-3334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  phosphopeptide non phosphopeptide |
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