| 中文名稱 | 谷氨酸受體調節蛋白2抗體 |
| 別 名 | BRCC1; NARG 2; NARG2_HUMAN; NMDA receptor regulated protein 2; NMDA receptor-regulated protein 2. |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 信號轉導 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 110kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NARG2:201-300/982 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | NARG2 is expressed at relatively high levels in dividing and immature cells, and is down-regulated upon terminal differentiation. NARG2 is a novel (S/T)PXX motif-containing nuclear protein; this motif is present in many transcription factors as well as other regulatory proteins that bind to DNA such as histones and RNA polymerase II. Three different isoforms exist. Subcellular Location: Nucleus (By similarity). Tissue Specificity: Expressed at low levels in lung and testis. SWISS: Q659A1 Gene ID: 79664 Database links: Entrez Gene: 79664 Human Entrez Gene: 93697 Mouse Omim: 610835 Human SwissProt: Q659A1 Human SwissProt: Q3UZ18 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Cerebrum (Rat) Lysate at 40 ug Raw264.7 (Mouse) Cell Lysate at 30 ug Primary: Anti- NARG2 (bs-5968R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARG2) Polyclonal Antibody, Unconjugated (bs-5968R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: U-2OS(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).  Blank control: RSC96(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).  Blank control: HCCLM3(blue)Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).  Blank control: U-2OS(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |
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