交叉反應 | Mouse, (predicted: Human, Rat, Chicken, Dog, Cow, Horse, Rabbit, ) |
產品介紹 | STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Subunit: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. Contains 1 SARAH domain. SWISS: Q9Y6E0 Gene ID: 8428 Database links: Entrez Gene: 8428 Human Entrez Gene: 223255 Mouse Entrez Gene: 361092 Rat Omim: 604984 Human SwissProt: Q9Y6E0 Human SwissProt: Q99KH8 Mouse SwissProt: B0LT89 Rat Unigene: 508514 Human Unigene: 390756 Mouse Unigene: 442081 Mouse Unigene: 472866 Mouse Unigene: 105397 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 產品圖片 | Sample: Muslce (Mouse) Lysate at 40 ug Fetal brain (Mouse) Lysate at 40 ug Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- MST3/STK3 (bs-7599Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 52 kD Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 53 kD Sample: Heart (Mouse) Lysate at 40 ug Bladder (Mouse) Lysate at 40 ug Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 53 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MST3 STK3) Polyclonal Antibody, Unconjugated (bs-7599R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-STK3/MST3 Polyclonal Antibody, Unconjugated(bs-7599R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining | |