| 中文名稱 | 血管生成素-1抗體 |
| 別 名 | ANG; AGP 1; AGP1; AGPT; ANG 1; ANG1; Angiopoietin1; Angiogenin; ANGPT 1; ANGPT1; ANGPT-1; Angiopoietin-1; Angiopoietin 1; ANG-1; angiopoietin-1 isoform 1 precursor; ANGP1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Angiopoietin 1:276-375/498 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]. Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Subunit: Homooligomer. Interacts with TEK/TIE2. Subcellular Location: Secreted. Post-translational modifications: Glycosylated. Similarity: Contains 1 fibrinogen C-terminal domain. SWISS: Q15389 Gene ID: 284 Database links: Entrez Gene: 284 Human Entrez Gene: 11600 Mouse Omim: 601667 Human SwissProt: Q15389 Human SwissProt: O08538 Mouse Unigene: 369675 Human Unigene: 309336 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Ang1/2與血管生成關(guān)系最為密切,目前有關(guān)該家族成員在腫瘤血管生成中的研究正日益增多。近年來(lái)也用于糖尿病導(dǎo)致的血管損傷研究也是熱門課題。 |
| 產(chǎn)品圖片 |  Sample:Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: U87MG (Human) Cell Lysate at 30 ug Lane 3: Heart (Mouse) Lysate at 40 ug Lane 4: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Angiopoietin-1 (bs-0800R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD  Sample:Lane 1: K562 (Human) Cell Lysate at 30 ug Lane 2: U87MG (Human) Cell Lysate at 30 ug Lane 3: Hela (Human) Cell Lysate at 30 ug Lane 4: Huvec (Human) Cell Lysate at 30 ug Primary: Anti-Angiopoietin-1 (bs-0800R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP94) Polyclonal Antibody, Unconjugated (bs-0194R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AGP 1 Polyclonal Antibody, Unconjugated(bs-0800R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ANGPTL1/Angiopoietin 1/ANG-1 Polyclonal Antibody, Unconjugated(bs-0800R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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