| 中文名稱 | 褪黑素受體1B抗體 |
| 別 名 | MT2; melatonin receptor type 1B; melatonin receptor; MEL1B; MEL-1B-R; Mtnr1b; MGC129286;MGC129287; MTNR-1B; Melatonin receptor 1B; MTR1B_HUMAN. |
| 研究領域 | 細胞生物 免疫學 神經生物學 生長因子和激素 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTNR1B:201-300/362 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. Function: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in retina and less in brain and hippocampus. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49286 Gene ID: 4544 Database links: Entrez Gene: 4544 Human Omim: 600804 Human SwissProt: P49286 Human Unigene: 569039 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 有學者認為：MTNR1B是組成控制褪黑激素信號通路的一部分。 褪黑激素調解人體的晝夜節律，是通過白天和黑夜來控制睡眠和飲食的內部時鐘。MTNR1B的進一步研究為高血糖和糖尿病與晝夜節律遭到破壞有直接關系這一觀點提供了證據。 |
| 產品圖片 |  Sample:Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Lane 7: Hela (Human) Cell Lysate at 30 ug Lane 8: U87MG (Human) Cell Lysate at 30 ug Primary: Anti-MTNR1B (bs-0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50-55 kD Observed band size: 60/50 kD  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti- MTNR1B (bs-0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (bs-0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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