| 中文名稱 | 抑制素/腫瘤抑制因子抗體 |
| 別 名 | Mitochondrial Marker; PHB; prohibitin; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 染色質(zhì)和核信號 細胞凋亡 細胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Prohibitin:171-272/272 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. Subunit: Interacts with PHB2. Interacts with STOML2. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Widely expressed in different tissues. Similarity: Belongs to the prohibitin family. SWISS: P35232 Gene ID: 5245 Database links: Entrez Gene: 5245 Human Entrez Gene: 18673 Mouse Entrez Gene: 25344 Rat Omim: 176705 Human SwissProt: P35232 Human SwissProt: P67778 Mouse SwissProt: P67779 Rat Unigene: 514303 Human Unigene: 263862 Mouse Unigene: 29754 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Prohibitin(phb)基因是一種有效的腫瘤抑制基因，廣泛分布于不同種屬的各種生物細胞中，它所編碼的產(chǎn)物Prohibitin(PHB)蛋白結(jié)構(gòu)保守，既存在于線粒體內(nèi)膜上，發(fā)揮分子伴侶作用，也存在于細胞核內(nèi)，具有負(fù)性轉(zhuǎn)錄調(diào)控作用，因而對細胞代謝、生長、分化、衰老以及凋亡等諸多方面發(fā)揮 著重要的調(diào)控作用，可能與某些腫瘤和退行性疾病的發(fā)生有關(guān)。 |
| 產(chǎn)品圖片 |  Sample:Heart(Mouse) Lysate at 40 ug Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD  Sample:A431(Human) Cell Lysate at 40 ug Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD  Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control:A549.Primary Antibody (green line): Rabbit Anti-Prohibitin antibody (bs-1449R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution:0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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