| 中文名稱 | CD38抗體 |
| 別 名 | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38_HUMAN; CD38_MOUSE; I-19; NIM-R5 antigen. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 干細(xì)胞 糖尿病 細(xì)胞表面分子 自然殺傷細(xì)胞 淋巴細(xì)胞 t-淋巴細(xì)胞 b-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD38:101-200/304 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Function: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Similarity: Belongs to the ADP-ribosyl cyclase family. SWISS: P56528 Gene ID: 12494 Database links: Entrez Gene: 952 Human Entrez Gene: 12494 Mouse Omim: 107270 Human SwissProt: P28907 Human SwissProt: P56528 Mouse Unigene: 479214 Human Unigene: 249873 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD38分子是單鏈Ⅱ型跨膜糖蛋白，存在于很多免疫細(xì)胞表面上,CD38與無脊椎動物卵的授精、HIV感染、某些癌癥以及糖尿病等有關(guān)，CD38具有許多復(fù)雜而又獨特的生物學(xué)特性及功能，有調(diào)控細(xì)胞鈣釋放的機(jī)制。主要表達(dá)于胸腺細(xì)胞、前T和前B細(xì)胞、活化的T和B細(xì)胞、單核細(xì)胞、NK細(xì)胞、漿細(xì)胞等，可用于急性白血病的分型和活化的T細(xì)胞在自身免疫和免疫缺陷中的作用等方面的研究，也可作為生發(fā)中心B細(xì)胞的一種選擇性標(biāo)記。 |
| 產(chǎn)品圖片 |  Sample:Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD  Sample:SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 54 kD  Sample:Pancreas (Mouse) Lysate at 40 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD38 Polyclonal Antibody, Unconjugated(bs-0979R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: mouse spleen cells (blue).Primary Antibody: Rabbit Anti- CD38 antibody(bs-0979R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-0979R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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