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胰高血糖素受體抗體

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中文名稱(chēng) 胰高血糖素受體抗體
別    名 GCGR; GGR; GL R; GL-R; GLR; GLR_HUMAN; MGC138246; Glucagon receptor precursor.  

 

研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞膜受體  糖尿病  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Guinea Pig, )
產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Glucagon Receptor:11-110/477 
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 The physiologic effects of glucagon are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase. The glucagon receptor has been reported to be expressed in liver and adipose, but not in adrenal medulla. ESTs have been isolated from liver/spleen and kidney libraries.

Function:
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P47871

Gene ID:
2642

Database links:

Entrez Gene: 2642 Human

Entrez Gene: 14527 Mouse

Entrez Gene: 24953 Rat

Omim: 138033 Human

SwissProt: P47871 Human

SwissProt: Q61606 Mouse

SwissProt: P30082 Rat

Unigene: 208 Human

Unigene: 22329 Mouse

Unigene: 11225 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片 Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptor (bs-3945R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptor(bs-3945R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (bs-3945R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

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