| 中文名稱 | G蛋白偶聯受體GPR91蛋白抗體 |
| 別 名 | G protein coupled receptor 91; G-protein coupled receptor 91; GPR 91; P2Y purinoceptor 1;P2Y purinoceptor 1-like; Purinergic Receptor P2U2; Succinate receptor; Succinate receptor 1; SUCN R1; SUCNR 1; SUCNR1; SUCR1_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR91:131-334/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009] Function: Receptor for succinate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed specifically in kidney. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9BXA5 Gene ID: 56670 Database links: Entrez Gene: 56670 Human Entrez Gene: 84112 Mouse Entrez Gene: 408199 Rat Omim: 606381 Human SwissProt: Q9BXA5 Human SwissProt: Q99MT6 Mouse SwissProt: Q6IYF9 Rat Unigene: 279575 Human Unigene: 125110 Mouse Unigene: 124512 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample: HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-GPR91(bs-15392R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39kD Observed band size: 39kD  Protein: 1.kidney lyates(mo);2.liver lyates(mo);Primary: Rabbit Anti-GPR91 (bs-15392R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:39 kD Observed band size:49 kD  Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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