| 中文名稱 | His Tag標簽抗體 |
| 別 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag. |
| 產品類型 | 標簽抗體 |
| 研究領域 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Recombinant protein |
| 產品應用 | WB=1:2000-5000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from His Tag: |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:His-tagged FMDV VP1 protein (type O) at 30 ug Primary: Anti-His Tag (bs-10582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 47 kD  Sample:His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug Primary: Anti-His Tag (bs-10582R) at 1/2000 ~ 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample:His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2 ug Primary: Anti-His Tag (bs-10582R) at 1/2000 ~ 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD  Sample:Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-His tag (bs-10582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD  Sample:Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bs-10582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD |
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