| 中文名稱 | 腺苷受體A3抗體 |
| 別 名 | adenosine A3 receptor; A3AR; AD 026; AD026; ADORA 3; ADORA3; bA552M11.5; RP11 552M11.7; TGPCR1; adenosine receptor A3 isoform 1; adenosine receptor A3 isoform 2; AA3R_HUMAN. |
| 研究領域 | 心血管 神經(jīng)生物學 細胞膜受體 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADORA3:85-180/347 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. Subcellular Location: Cell membrane. Post-translational modifications: Phosphorylation on Ser-317 may be crucial for rapid desensitization. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P33765 Gene ID: 140 Database links: Entrez Gene: 403805 Dog Entrez Gene: 140 Human Entrez Gene: 606753 Pig Omim: 600445 Human SwissProt: Q28309 Dog SwissProt: P33765 Human SwissProt: Q6P2N6 Human Unigene: 281342 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ADORA-3(腺苷受體A3)屬于G蛋白偶聯(lián)受體家族，在腺苷調節(jié)細胞活力和生長中起關鍵作用，并且在很多腫瘤中表達增高，A3AR可能成為腫瘤診斷標志物和治療靶點。 |
| 產(chǎn)品圖片 |  Sample:Colon carcinoma lysates (Human) Lysate at 40 ug Gastric carcinoma lysates (Human) Lysate at 40 ug Primary: Anti- ADORA3 (bs-1225R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 35 kD Observed band size: 35 kD  Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated(bs-1225R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-1225R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃).Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(bs-1225R); Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test. |
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