| 中文名稱 | 神經(jīng)生長(zhǎng)相關(guān)蛋白43抗體 |
| 別 名 | Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Dog, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:200-800 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25/46kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GAP43:9-100/238 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Subunit: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes. Post-translational modifications: Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. Similarity: Belongs to the neuromodulin family. Contains 1 IQ domain. SWISS: P06837 Gene ID: 2596 Database links: Entrez Gene: 2596 Human Entrez Gene: 14432 Mouse Entrez Gene: 29423 Rat GenBank: NP_002036 Human Omim: 162060 Human SwissProt: P17677 Human SwissProt: P06837 Mouse SwissProt: P07936 Rat Unigene: 134974 Human Unigene: 1222 Mouse Unigene: 10928 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology）；神經(jīng)標(biāo)志物 GAP43 （Growth associated protein-43）又稱作neuromodulin，是一個(gè)軸突膜蛋白，是一種神經(jīng)特異性的蛋白質(zhì)，參與神經(jīng)細(xì)胞外生長(zhǎng)及突觸發(fā)育形成和神經(jīng)細(xì)胞再生。在神經(jīng)元發(fā)育和再生過(guò)程中以高水平表達(dá)。能調(diào)解軸突延伸作用，改變細(xì)胞形態(tài)。作為細(xì)胞內(nèi)信號(hào)，可大大增強(qiáng)與G蛋白偶聯(lián)的受體轉(zhuǎn)運(yùn)作用。 神經(jīng)生長(zhǎng)相關(guān)蛋白-GAP-43和神經(jīng)細(xì)胞黏附因子(neural cell adhesion molecule,NCAM)與突觸可塑性密切相關(guān).GAP-43是一種神經(jīng)細(xì)胞膜上的特異性磷蛋白,在神經(jīng)發(fā)育和再生過(guò)程中呈現(xiàn)高表達(dá),被作為突觸生長(zhǎng)的標(biāo)志物,有稱脊髓生長(zhǎng)相關(guān)蛋白 GAP-43與CaM結(jié)合,參與G蛋白相互作用,神經(jīng)遞質(zhì)的釋放,作用于胞吞/胞吐過(guò)程,通過(guò)小囊溶合或誘導(dǎo)生長(zhǎng)錐和突觸前末端的胞吞促進(jìn)膜擴(kuò)展,與海馬長(zhǎng)時(shí)程增強(qiáng)密切相關(guān). 神經(jīng)細(xì)胞黏附因子是細(xì)胞表面糖蛋白大家族的成員之一,促進(jìn)軸突生長(zhǎng),對(duì)長(zhǎng)時(shí)記憶的保持有重要影響,同時(shí),GAP-43對(duì)其具調(diào)節(jié)作用，廣泛而深入地研究GAP-43意義深遠(yuǎn)。 |
| 產(chǎn)品圖片 | Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GAP-43 Polyclonal Antibody, Unconjugated(bs-0154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Blank control (blue line): Hela cells (blue).Primary Antibody (green line): Rabbit Anti-GAP43 antibody (bs-0154R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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