| 中文名稱 | 基質金屬蛋白酶-1抗體 |
| 別 名 | 27 kDa interstitial collagenase; CLGN; CLG; collagenase, fibroblast; Fibroblast collagenase; Interstitial collagenase; MMP 1; MMP-1; MMP; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix metalloproteinase-1; MMP1_HUMAN; OTTHUMP00000045866; Matrix Metalloproteinase 1. |
| 研究領域 | 腫瘤 心血管 信號轉導 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rabbit, (predicted: Cow, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27/41/54kDa |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP1:401-469/469 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009] Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Subunit: Interacts with HIV-1 Tat. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Post-translational modifications: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P03956 Gene ID: 4312 Database links: Entrez Gene: 281308 Cow Entrez Gene: 4312 Human Entrez Gene: 17386 Mouse Entrez Gene: 397320 Pig Entrez Gene: 100009110 Rabbit Omim: 120353 Human SwissProt: P28053 Cow SwissProt: P03956 Human SwissProt: P33435 Mouse SwissProt: P21692 Pig SwissProt: P13943 Rabbit Unigene: 83169 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成與降解（Synthesis and Degradation） 基質金屬蛋白酶(matrix metalloproteinases, MMPs)是一族依賴鋅離子而降解各種細胞外基質的蛋白酶，亦稱IV型膠原酶或稱明膠酶A，其主要功能為降解IV型膠原，因而它在腫瘤細胞突破基底膜屏障和浸潤轉移中起重要作用。 目前主要用于各種惡性腫瘤(如乳腺癌、胃腸道癌、卵巢癌、膀胱癌等)中的基底膜檢測與腫瘤轉移浸潤的研究。 |
| 產品圖片 |  Sample: Placenta (Mouse) Lysate at 30 ugPrimary: Anti- MMP-1 (bs-0424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD  Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-MMP-1 (bs-0424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/54kD Observed band size: 50kD  Sample: Heart (Rabbit) Lysate at 30 ugPrimary: Anti-MMP-1 (bs-0424R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 24 kD Observed band size: 27 kDTissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-1 Polyclonal Antibody, Unconjugated(bs-0424R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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