| 中文名稱 | 磷酸化纖維束蛋白同源物1抗體 |
| 別 名 | Fascin (phospho Ser39); Fascin (phospho S39); 55 kDa actin bundling protein; Actin bundling protein; FAN 1; FAN1; Fascin 1; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like protein; SNL; Strongylocentrotus purpuratus. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 信號轉導 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Dog, Pig, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3μg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39:AS(p-S)LK |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells. Function: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Subcellular Location: Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. Similarity: Belongs to the fascin family. SWISS: Q16658 Gene ID: 6624 Database links: Entrez Gene: 6624 Human Entrez Gene: 14086 Mouse Entrez Gene: 683788 Rat Omim: 602689 Human SwissProt: Q16658 Human SwissProt: Q61553 Mouse SwissProt: P85845 Rat Unigene: 118400 Human Unigene: 289707 Mouse Unigene: 199526 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 肌動蛋白集束蛋白/圓線蟲紫癜抗體。 FSCN1為細胞骨架肌動蛋白的一種，該蛋白有蛋白結合,橋連、肌動蛋白絲結合的功能。 主要參與細胞增殖、肌動蛋白細胞骨架組織和生物發生、肌動蛋白絲束形成。< |
| 產品圖片 |  Sample:U87MG(Human) Cell Lysate at 30 ug Primary: Anti-phospho-FSCN1(Ser39) (bs-0772R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSCN1(Ser39)) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: musle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated(bs-0772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: U937(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green). |
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