| 中文名稱 | T淋巴細胞負調節蛋白抗體 |
| 別 名 | V-set and immunoglobulin domain-containing protein 4; CRIg; V set and immunoglobulin domain containing 4; Z39IG; VSIG4_HUMAN. |
| 研究領域 | 免疫學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Dog, Cow, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VSIG4:81-160/399 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | T cell activation by APCs is positively and negatively regulated by members of the B7 family. We have identified a previously unknown function for B7 familyrelated protein V-set and Ig domaincontaining 4 (VSIG4). Administration to mice of soluble VSIG4-Ig fusion molecules reduced the induction of T cell responses in vivo and inhibited the production of Th celldependent IgG responses. Unlike that of B7 family members, surface expression of VSIG4 was restricted to resting tissue macrophages and absent upon activation by LPS or in autoimmune inflammatory foci. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues. Function: Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. Subcellular Location: Membrane; Single-pass type I membrane protein (Probable). Tissue Specificity: Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages. Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q9Y279 Gene ID: 11326 Database links: Entrez Gene: 11326 Human Entrez Gene: 278180 Mouse Entrez Gene: 312102 Rat Omim: 300353 Human SwissProt: Q9Y279 Human Unigene: 8904 Human Unigene: 26781 Mouse Unigene: 138093 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (bs-0479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Lung(Mouse)Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Primary: Anti-VSIG4 (bs-0479R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (bs-0479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti- VSIG4 Polyclonal Antibody, Unconjugated(bs-0479R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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