| 中文名稱 | 磷酸二酯酶4D抗體 |
| 別 名 | cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; FLJ97311; cAMP specific 3' 5' cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D; cAMP specific phosphodiesterase PDE4D6; cyclic AMP phosphodiesterase; DPDE 3; DPDE3; Dunce like phosphodiesterase E3; HSPDE4D; PDE 43; PDE43; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); Phosphodiesterase E3 dunce homolog Drosophila; STRK 1; STRK1. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 90kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDE4D:401-550/809 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein protein interactions with cytoskeletal scaffolding proteins. Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Subunit: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Subcellular Location: Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. Tissue Specificity: Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Post-translational modifications: Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. SWISS: Q08499 Gene ID: 5144 Database links: Entrez Gene: 5144 Human Entrez Gene: 238871 Mouse Entrez Gene: 24627 Rat Omim: 600129 Human SwissProt: Q08499 Human SwissProt: P14270 Rat Unigene: 117545 Human Unigene: 95959 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 經(jīng)研究發(fā)現(xiàn)磷酸二酯酶(PDE4D）基因可使缺血性卒中的危險(xiǎn)性增高,使人患動(dòng)脈粥樣硬化及中風(fēng)的風(fēng)險(xiǎn)性增高,有可能是通過影響動(dòng)脈內(nèi)平滑肌細(xì)胞的增生和擴(kuò)散相關(guān)。目前，ＰＤＥ４Ｄ基因的變化機(jī)理并不是完全清楚，有待進(jìn)一步研究 |
| 產(chǎn)品圖片 |  Sample:Brain(Mouse) Lysate at 30 ugPrimary: Anti-PDE4D (bs-1325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 100 kD  Sample:Brain(Mouse) Lysate at 30 ugPrimary: Anti-PDE4D (bs-1325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- PDE4D (bs-1325R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90 kD  Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green). |
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