研究領域腫瘤  細胞生物  免疫學  神經生物學  生長因子和激素  內分泌病  結合蛋白  

抗體來源Rabbit

克隆類型Polyclonal

交叉反應Mouse, Rat,  (predicted: Human, Dog, Cow, Rabbit, )

產品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量40kDa

細胞定位分泌型蛋白 

性    狀Liquid

濃    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human ABP:51-150/402 

亞    型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

產品介紹Sex hormone binding globulin (SHBG) is a glycoprotein synthesized by the liver. Circulating androgen and estrogen concentrations influence SHBG synthesis. Elevated testosterone, for example, causes SHBG synthesis to decrease, whereas high estrogen stimulates SHBG production. The regulation of SHBG synthesis, combined with SHBG's higher affinity for testosterone, impacts bioavailable testosterone levels. SHBG binds up to 98 percent of the steroid hormones in the blood including 5a-dihydrotestosterone (DHT), testosterone and androstenediol with particularly high affinity, and estradiol and estrone with slightly lower affinity.

Function:

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Subunit:

Homodimer.

Subcellular Location:

Secreted (By similarity). Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis (By similarity).

Tissue Specificity:

Isoform 1 and isoform 2 are present in liver and testis.

Similarity:

Contains 2 laminin G-like domains.

SWISS:

P04278

Gene ID:

6462

Database links:

Entrez Gene: 404182 Cow

Entrez Gene: 6462 Human

Omim: 182205 Human

SwissProt: P04278 Human

Unigene: 632235 Human

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

雄激素結合蛋白ABP是一種由睪丸支持細胞合成和分泌的與雄激素有高度親和力的蛋白,雄激素是依賴于雄激素結合蛋白起作用的,ABP與睪酮或雙氫睪酮結合后，轉運至曲細精管，提高雄激素在曲細精管的局部濃度，有利于生精過程。

產品圖片

Sample: Lane1: Testis (Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-ABP/SHBG(bs-2410R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 44kD Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.