研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞膜受體  

抗體來源Rabbit

克隆類型Polyclonal

交叉反應(yīng)Human, Mouse,  (predicted: Rat, )

產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量58kDa

細(xì)胞定位細(xì)胞漿 細(xì)胞膜 

性    狀Liquid

濃    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human CD15:251-295/433 

亞    型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

產(chǎn)品介紹The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq].

Function:

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Subcellular Location:

Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.

Tissue Specificity:

Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas.

Similarity:

Belongs to the glycosyltransferase 10 family.

SWISS:

Q11127

Gene ID:

2526

Database links:

Entrez Gene: 10690 Human

Entrez Gene: 2526 Human

Entrez Gene: 2527 Human

Entrez Gene: 2528 Human

Entrez Gene: 2529 Human

Omim: 104230 Human

SwissProt: P22083 Human

SwissProt: P51993 Human

SwissProt: Q11128 Human

SwissProt: Q11130 Human

SwissProt: Q9Y231 Human

Unigene: 390420 Human

Unigene: 457 Human

Unigene: 49117 Human

Unigene: 572064 Human

Unigene: 623098 Human

Unigene: 631843 Human

Unigene: 631846 Human

Unigene: 705615 Human

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD15(SSEA-1)是胚胎干細(xì)胞表達(dá)胚胎階段特異性抗原的主要SSEA-1蛋白,還包括、SSEA-3、TRA-1-81、TRA-1-60等。CD15主要存在于粒細(xì)胞（包括中性粒細(xì)胞和嗜酸性粒細(xì)胞）以及部分單核細(xì)胞上。它在介導(dǎo)細(xì)胞吞噬細(xì)菌和趨化性方面起重要作用。CD15常表達(dá)于霍奇金氏病中R-S細(xì)胞，尤其是經(jīng)典型霍奇金淋巴瘤中。結(jié)節(jié)硬化型霍奇金淋巴瘤多為CD15陽性。CD15抗體是鑒定霍奇金氏病的一個(gè)有效工具。

產(chǎn)品圖片

Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-CD15(bs-1702R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kD Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD15) Polyclonal Antibody, Unconjugated (bs-1702R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Overlay histogram showing HL 60 cells stained with bs-1702R (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-1702R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD15 /FITC Conjugated antibody (bs-1702R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.