中文名稱白介素-1受體拮抗劑抗體
別 名Interleukin-1 receptor antagonist; ICIL 1RA; IL1 inhibitor; ICIL1RA; IL1F3; IL1RN; IRAP; IL1RA_HUMAN.
研究領(lǐng)域免疫學
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Sheep, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量17kDa
細胞定位細胞漿 分泌型蛋白
性 狀Liquid
濃 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human IL-1RA:26-120/178
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Function:
Inhibits the activity of interleukin-1 by binding to eceptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Subcellular Location:
Isoform 1: Secreted.
Isoform 2, 3, 4: Cytoplasm.
Tissue Specificity:
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
DISEASE:
Microvascular complications of diabetes 4 (MVCD4) [MIM:612628]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]: A rare autoinflammatory disease of skin and bone resulting in sterile multifocal osteomyelitis, periostitis, and pustulosis from birth. The term autoinflammatory disease describes a group of disorders characterized by attacks of seemingly unprovoked inflammation without significant levels of autoantibodies and autoreactive T-cells. Note=The disease is caused by mutations affecting the gene represented in this entry.
SWISS:
P25085
Gene ID:
3557
Database links:
Entrez Gene: 3557 Human
Entrez Gene: 16181 Mouse
Entrez Gene: 60582 Rat
Omim: 147679 Human
SwissProt: P18510 Human
SwissProt: P25085 Mouse
SwissProt: P25086 Rat
Unigene: 81134 Human
Unigene: 882 Mouse
Unigene: 162640 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
IL-1受體拮抗劑(IL-1ra)是IL-1強有力的內(nèi)生拮抗劑，通過與白細胞介素-1的Ⅰ型和Ⅱ型受體結(jié)合，特異性地阻斷白細胞介素-1的生物學作用。白介素1受體拮抗劑(IL-1Ra)是體內(nèi)天然的一種對IL-1有拮抗作用的細胞因子。
| 產(chǎn)品圖片 |  Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD  Sample: Spleen(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD  Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (bs-2216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(bs-2216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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