| 中文名稱 | CD9蛋白抗體 |
| 別 名 | Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal; 602 29; Antigen defined by monoclonal; 60229; BA 2; BA2; BTCC 1; BTCC1; CD 9; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-inhibiting gene 2 protein; DRAP 27; DRAP27; GIG 2; GIG2; Growth inhibiting gene 2 protein; Leukocyte antigen MIC3; MIC 3; MIC3; Motility related protein; Motility-related protein; MRP 1; MRP-1; MRP1; p24; P24; p24 antigen; Tetraspanin 29; Tetraspanin-29; Tetraspanin29; Tspan 29; Tspan-29; Tspan29. |
| 研究領域 | 腫瘤 細胞生物 免疫學 發育生物學 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD9:101-200/228 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Subunit: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed by a variety of hematopoietic and epithelial cells. Post-translational modifications: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Similarity: Belongs to the tetraspanin (TM4SF) family. SWISS: P21926 Gene ID: 928 Database links: Entrez Gene: 928 Human Entrez Gene: 12527 Mouse Entrez Gene: 24936 Rat Omim: 143030 Human SwissProt: P21926 Human SwissProt: P40240 Mouse SwissProt: P40241 Rat Unigene: 114286 Human Unigene: 210676 Mouse Unigene: 404614 Mouse Unigene: 2091 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD9是一種普遍存在于細胞膜表面屬于四跨膜超蛋白家族的細胞表面糖蛋白, 參與質膜的融合過程，在細胞膜生物學中發揮重要作用。 近年來經研究發現，CD9與多種腫瘤轉移有關，故稱腫瘤轉移抑制基因亦稱抗腫瘤轉移基因。是一個新的腫瘤轉移抑制基因膜運動相關蛋白-1(CD9/MRP-1)，其分子功能還有待于進一步研究。CD9主要在早B細胞、活化T淋巴細胞、嗜酸性粒細胞、嗜堿性粒細胞和血小板表達。 |
| 產品圖片 |  Sample:Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Placenta (Mouse) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Primary: Anti-CD9 (bs-2489R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD  Blank control: Mouse spleen cells(blue).Primary Antibody:Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.  Blank control: RSC96 cells (blue).Primary Antibody:Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.  Blank control: Raji(blue).Primary Antibody: Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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