Entrez Gene: 3164 Human Entrez Gene: 15370 Mouse Entrez Gene: 79240 Rat Omim: 139139 Human SwissProt: P22736 Human SwissProt: P12813 Mouse SwissProt: P22829 Rat Unigene: 524430 Human Unigene: 119 Mouse Unigene: 10000 Rat
中文名稱 孤兒核受體蛋白Nur77抗體 別 名 GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; NGFIB; NP 10; NP10; NR4A1; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear Receptor Subfamily 4 Group A Member 1; NUR77; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; ST 59; ST59; Steroid receptor TR3; TR 3; TR3; TR3 orphan receptor; Early response protein NAK1. 研究領域 腫瘤 細胞生物 免疫學 染色質和核信號 神經生物學 信號轉導 細胞凋亡 轉錄調節因子 糖尿病 抗體來源 Rabbit 克隆類型 Polyclonal 交叉反應 Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) 產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 66kDa 細胞定位 細胞核 性 狀 Liquid 濃 度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human Nur77/GFRP:351-450/598 亞 型 IgG 純化方法 affinity purified by Protein A 儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 產品介紹 This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Subunit:
Binds DNA as a monomer (By similarity). Interacts with GADD45GIP1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Fetal muscle and adult liver, brain and thyroid.
Similarity:
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
P22736
Gene ID:
3164
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
孤兒核受體蛋白Nur77是早期反應轉錄因子之一，多種刺激因子如缺氧可誘導其迅速表達，Nur77參與調控細胞增殖、存活、分化等過程。這類核受體是體內廣泛分布的配體依賴性的轉錄因子，通過調控基因協調表達，在機體的生長發育、新陳代謝，細胞分化及體內許多生長發育過程中發揮重要作用，其功能障礙將導致一系列疾病的發生，如；癌癥、糖尿病、肥胖、不孕不育等等。 產品圖片 
Sample:
Muscle(Mouse) Lysate at 40 ug
Primary: Anti-Nur77(bs-3513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 66 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nur77 Polyclonal Antibody, Unconjugated(bs-3513R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Nur77 antibody (bs-3513R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
