Entrez Gene: 369 Human Entrez Gene: 64363 Rat Entrez Gene: 403063 Zebrafish Omim: 311010 Human SwissProt: P10398 Human SwissProt: P14056 Rat Unigene: 446641 Human Unigene: 1714 Rat
中文名稱(chēng) A-Raf抗體 別 名 A raf 1; A-Raf; Raf1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 (murine sarcoma 3611 virus). 研究領(lǐng)域 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 抗體來(lái)源 Rabbit 克隆類(lèi)型 Polyclonal 交叉反應(yīng) Human, Mouse, Rat, 產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 68kDa 細(xì)胞定位 細(xì)胞漿 性 狀 Liquid 濃 度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human A Raf/ARAF:151-250/606 亞 型 IgG 純化方法 affinity purified by Protein A 儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 產(chǎn)品介紹 The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Function:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue Specificity:
Predominantly in urogenital tissues.
Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
SWISS:
P10398
Gene ID:
369
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片 
Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-A Raf (bs-2251R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 65 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-A Raf (bs-2251R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (bs-2251R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (bs-2251R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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