日本精久视频69视频在线播放_国产精品狼人色视频一区_1024手机基地你懂的日韩人妻_急救护士美国满天星全名_亚洲狼人香蕉香蕉在线28_亚洲一级电影在线观看_国产a国产高清免费_亚洲情s网站大全_日韩美女日批爽爽爽_欧美三级一区二区在线观看

您好,歡迎光臨上海雅吉生物商城!
工作時(shí)間:9:00-18:00
全國(guó)服務(wù)熱線:021-34661276

羥類固醇脫氫酶17β3抗體

訂購(gòu)數(shù)量:
規(guī)格
價(jià)格庫(kù)存
訂購(gòu)熱線:021-34661276
我要詢價(jià)
  • 商品詳情
  • 售后服務(wù)
  • 相關(guān)文獻(xiàn)

 

中文名稱 羥類固醇脫氫酶17β3抗體
別    名 17 beta HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; EDH17B3; Estradiol 17 beta dehydrogenase; Estradiol 17 beta dehydrogenase 3; Hydroxysteroid (17 beta) dehydrogenase 3; Testicular 17 beta hydroxysteroid dehydrogenase; Testosterone 17 beta dehydrogenase 3; DHB3_HUMAN.  

 

研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  發(fā)育生物學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 34kDa
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HSD17B3:101-200/310 
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 HSD17B3 is involved in the reduction of androstenedione to testosterone. It is expressed predominantly in the testis. Deficiency in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH).

Function:
Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.

Tissue Specificity:
Testis.

DISEASE:
Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH) [MIM:264300]. These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place.

Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

SWISS:
P37058

Gene ID:
3293

Database links:

Entrez Gene: 3293 Human

Omim: 605573 Human

SwissProt: P37058 Human

Unigene: 477 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片 Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HSD17B3 (bs-3905R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSD17B3 Polyclonal Antibody, Unconjugated(bs-3905R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD17B3) Polyclonal Antibody, Unconjugated (bs-3905R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-HSD17B3 antibody (bs-3905R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
我要詢價(jià)
*聯(lián)系方式:
(可以是QQ、MSN、電子郵箱、電話等,您的聯(lián)系方式不會(huì)被公開(kāi))
*內(nèi)容:


微信客服
掃一掃立即咨詢


微信客服
掃一掃立即咨詢

銷售電話:

021-34661275

021-34661276

15301693058