| 中文名稱 | 快速骨骼肌肌球蛋白輕鏈2抗體 |
| 別 名 | 2410014J02Rik; DKFZp779C0757; DTNB; Fast skeletal myosin light chain 2; G2; HUMMLC2B; MGC13450; MLC 2; MRLC2; MYL11; MLC2B; MLC2F; MLRS_HUMAN; MYLPF; Myosin light polypeptide 2 alkali; Myosin regulatory light chain 2, skeletal muscle isoform. |
| 研究領域 | 免疫學 信號轉導 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fast skeletal myosin light chain 2:101-170/170 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Tissue Specificity: Expressed in fetal and adult skeletal muscle. Similarity: Contains 3 EF-hand domains. SWISS: Q96A32 Gene ID: 29895 Database links: Entrez Gene: 29895 Human Entrez Gene: 396470 Chicken Entrez Gene: 317657 Cow Entrez Gene: 4632 Human Entrez Gene: 17901 Mouse Entrez Gene: 17907 Mouse Entrez Gene: 24584 Rat Entrez Gene: 56781 Rat Omim: 160780 Human SwissProt: P02609 Chicken SwissProt: P05976 Human SwissProt: P06741 Human SwissProt: Q14324 Human SwissProt: P05978 Mouse SwissProt: P97457 Mouse SwissProt: P02602 Rabbit SwissProt: P02603 Rabbit SwissProt: P02600 Rat SwissProt: P04466 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Muscle (Mouse) Lysate at 40 ug Primary: Anti-MYLPF (bs-5159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD  Sample:A673(Human) Cell Lysate at 30 ug Primary: Anti-MYLPF (bs-5159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD  Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(bs-5159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (bs-5159R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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