| 中文名稱 | 磷酸化原癌基因蛋白18抗體 |
| 別 名 | Stathmin 1 (phospho S63); p-Stathmin 1 (phospho S63); Stathmin 1 (Phospho-Ser63); Stathmin (Phospho Ser63); Stathmin (Phospho S63); Lag; LAP 18; LAP18; Leukemia associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; OP18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; PP17; PP19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin1; STMN 1; STMN1; STMN1_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞粘附分子 細(xì)胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Cow, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:50-200 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 16kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser63:41-140/149 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] Function: Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. Subunit: Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. Post-translational modifications: Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Similarity: Belongs to the stathmin family. SWISS: P16949 Gene ID: 3925 Database links: Entrez Gene: 3925 Human Entrez Gene: 16765 Mouse Entrez Gene: 29332 Rat Omim: 151442 Human SwissProt: P16949 Human SwissProt: P54227 Mouse SwissProt: P13668 Rat Unigene: 209983 Human Unigene: 378957 Mouse Unigene: 555 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 原癌基因蛋白18(oncogene protein 18,Op18),是一種廣泛存在于細(xì)胞質(zhì)的蛋白質(zhì),可與微管蛋白結(jié)合,參與微管和紡錘體的組裝;與細(xì)胞的增殖、分化、再生和運(yùn)動(dòng)均有關(guān),并具有信號(hào)活性調(diào)節(jié)的功能。在細(xì)胞增殖、分化的信號(hào)途徑、傳到通路激活中也具有多種調(diào)節(jié)功能。 近年來(lái)有研究發(fā)現(xiàn),stathmin在多種腫瘤中高表達(dá),并可通過(guò)調(diào)節(jié)微管的解聚,促進(jìn)腫瘤細(xì)胞的運(yùn)動(dòng)及侵襲。Stathmin翻譯后修飾狀態(tài)的改變,可影響與p53蛋白的相互作用,參與腫瘤的發(fā)生發(fā)展. |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Positive control: (mo)Thymocyte (2% Paraformaldehyde-fixed )Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-phospho-Stathmin 1 (Ser63) (bs-9764R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA |
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