| 中文名稱 | 14-3-3β抗體 |
| 別 名 | 14 3 3 protein beta/alpha; 14-3-3 protein beta/alpha; 1433B_HUMAN; Brain protein 14 3 3 beta isoform; GW 128; GW128; HS 1; HS1; KCIP 1; KCIP-1; KCIP1; N-terminally processed; Protein 1054; Protein kinase C inhibitor protein 1; Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide; YWHAA; YWHAB. |
| 研究領域 | 細胞生物 神經生物學 信號轉導 干細胞 細胞凋亡 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 14-3-3 beta:51-150/246 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | 14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II, and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It belongs to the 14-3-3 family. This antibody is reactive with 14-3-3 Alpha/Beta/Gamma/Delta/Epsilon. Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Subcellular Location: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: The alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1. Similarity: Belongs to the 14-3-3 family. SWISS: P31946 Gene ID: 7529 Database links: Entrez Gene: 7529 Human Entrez Gene: 54401 Mouse Entrez Gene: 56011 Rat Omim: 601289 Human SwissProt: P31946 Human SwissProt: Q9CQV8 Mouse SwissProt: P35213 Rat Unigene: 643544 Human Unigene: 34319 Mouse Unigene: 485025 Mouse Unigene: 8653 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Testis(Mouse) Lysate at 40 ug Primary: Anti-14-3-3 beta (bs-12420R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 beta) Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 beta) Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 beta) Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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