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磷酸化酪蛋白激酶1γ1+γ2+γ3抗體

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中文名稱 磷酸化酪蛋白激酶1γ1+γ2+γ3抗體
別    名 Casein kinase I isoforms gamma 1+2+3 (phospho Y263); p-CK1g2(phospho Y263); p-CSNK1G1(phospho Y263); p-CSNK1G2(phospho Y263); p-CSNK1G3(phospho Y263); Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3; KC1G1_HUMAN.  
產品類型 磷酸化抗體 
研究領域 細胞生物  信號轉導  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, )
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 49kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Casein kinase I isoforms gamma 1+2+3 around the phosphorylation site of Tyr263:ER(p-Y)QK 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. They can phosphorylate a large number of proteins. Participates in Wnt signaling.

Function:
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.

Subunit:
Monomer.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Autophosphorylated (By similarity).

Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.

SWISS:
Q9HCP0

Gene ID:
53944

Database links:

Entrez Gene: 53944 Human

Entrez Gene: 214897 Mouse

Omim: 606274 Human

SwissProt: Q9HCP0 Human

SwissProt: Q8BTH8 Mouse

Unigene: 646508 Human

Unigene: 325816 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產品圖片 Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CSNK1G 1+2+3 ( Tyr263)) Polyclonal Antibody, Unconjugated (bs-12426R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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