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HERC6 E3泛素蛋白連接酶抗體

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中文名稱 HERC6 E3泛素蛋白連接酶抗體
別    名 EC 6.3.2.; FLJ20637; HECT domain and RCC1 like domain containing protein 6; HECT domain and RCC1-like domain-containing protein 6; Hect domain and RLD 6; HERC 6; HERC6; HERC6_HUMAN; Potential ubiquitin ligase; Probable E3 ubiquitin protein ligase HERC6; Probable E3 ubiquitin-protein ligase HERC6.   
研究領域 細胞生物  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat,  (predicted: Human, Pig, Rabbit, )
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 115kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HERC6:751-850/1022 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹  
產品圖片 Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-HERC6(bs-15463R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kD
Observed band size: 115kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (bs-15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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