中文名稱 | HERC6 E3泛素蛋白連接酶抗體 |
別 名 | EC 6.3.2.; FLJ20637; HECT domain and RCC1 like domain containing protein 6; HECT domain and RCC1-like domain-containing protein 6; Hect domain and RLD 6; HERC 6; HERC6; HERC6_HUMAN; Potential ubiquitin ligase; Probable E3 ubiquitin protein ligase HERC6; Probable E3 ubiquitin-protein ligase HERC6. |
研究領域 | 細胞生物 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse, Rat, (predicted: Human, Pig, Rabbit, ) |
產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 115kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HERC6:751-850/1022 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產品介紹 | |
產品圖片 | Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti-HERC6(bs-15463R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115kD Observed band size: 115kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (bs-15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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