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FAM124A蛋白抗體

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中文名稱 FAM124A蛋白抗體
別    名 F124A_HUMAN; FAM124A; family with sequence similarity 124, member A; family with sequence similarity 124A; hypothetical protein LOC220108; Protein FAM124A.  
研究領域 細胞生物  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse,  (predicted: Rat, Chicken, Pig, Cow, Horse, Sheep, )
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 60kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FAM124A:301-400/546 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹  
產品圖片 Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60kD
Observed band size: 63kD
Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60kD
Observed band size: 60kD
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60kD
Observed band size: 63kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THOC1) Polyclonal Antibody, Unconjugated (bs-14712R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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