| 英文名稱 | FITM1 |
| 中文名稱 | FITM1蛋白抗體 |
| 別 名 | Fat inducing protein 1; Fat inducing transcript 1; Fat storage inducing transmembrane protein 1; Fat storage-inducing transmembrane protein 1; Fat-inducing protein 1; FIT1; FITM 1; Fitm1; FITM1_HUMAN; MGC46490. |
| 研究領域 | 細胞生物 免疫學 信號轉導 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 32kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FITM1:1-100/292 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | FIT1 belongs to an evolutionarily conserved family of proteins involved in fat storage (Kadereit et al., 2008 [PubMed 18160536]).[supplied by OMIM, May 2008] Function: Plays an important role in lipid droplet accumulation. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Primarily expressed in heart and skeletal muscle. Similarity: Belongs to the FIT family. SWISS: A5D6W6 Gene ID: 161247 Database links: Entrez Gene: 161247 Human Entrez Gene: 68680 Mouse Omim: 612028 Human SwissProt: A7YWN2 Cow SwissProt: A5D6W6 Human SwissProt: Q91V79 Mouse SwissProt: B2MVP8 Pig Unigene: 128060 Human Unigene: 36758 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Protein: 293T(human) lysate at 40ug;Primary: rabbit Anti-FITM1 (bs-16090R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 32 kD Observed band size: 28 kD  Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. |
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