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TRIT1蛋白抗體

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中文名稱 TRIT1蛋白抗體
別    名 2310075G14Rik; AI314189; AI314635; AV099619; FLJ20061; hGRO1; IPP transferase; IPPT; IPT; IPTase; Isopentenyl diphosphate:tRNA isopentenyltransferase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; MGC149242; MGC149243; mitochondrial; MOD5; MOD5_HUMAN; OTTMUSP00000009314; Putative protein TR2IT1; RP23-121J14.6; TRIT 1; TRIT1; tRNA dimethylallyltransferase; tRNA isopentenylpyrophosphate transferase; tRNA isopentenyltransferase 1; tRNA isopentenyltransferase; tRNA isopentenyltransferase mitochondrial.  
研究領域 細胞生物  信號轉導  轉錄調節因子  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TRIT1:121-220/467 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).

Subcellular Location:
Cytoplasm and Mitochondrion.

Similarity:
Belongs to the IPP transferase family.
Contains 1 matrin-type zinc finger.

SWISS:
Q9H3H1

Gene ID:
54802

Database links:

Entrez Gene: 54802 Human

Entrez Gene: 66966 Mouse

Entrez Gene: 362586 Rat

SwissProt: Q9H3H1 Human

SwissProt: Q80UN9 Mouse

Unigene: 356554 Human

Unigene: 235030 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產品圖片 Sample: Uterus(Mouse) Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD
Sample: Lung(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD
Sample: Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIT1) Polyclonal Antibody, Unconjugated (bs-17134R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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