| 英文名稱 | BMP2 |
| 中文名稱 | 骨形態發生蛋白2抗體 |
| 別 名 | Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN. |
| 研究領域 | 細胞生物 免疫學 發育生物學 神經生物學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 13/44kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP2:341-396/396 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. Function: Induces cartilage and bone formation. Subunit: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN. Subcellular Location: Secreted. Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. Similarity: Belongs to the TGF-beta family. SWISS: P12643 Gene ID: 650 Database links: Entrez Gene: 650 Human Entrez Gene: 12156 Mouse Entrez Gene: 29373 Rat Omim: 112261 Human SwissProt: P12643 Human SwissProt: P21274 Mouse SwissProt: P49001 Rat Unigene: 73853 Human Unigene: 103205 Mouse Unigene: 90931 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Stomach (Mouse) Lysate at 40 ug Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/44 kD Observed band size: 59 kD  Sample:Lane 1: U2os (Human) Cell Lysate at 30 ug Lane 2: MG63 (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Lane 5: THP-1 (Human) Cell Lysate at 30 ug Primary: Anti-BMP2 (bs-10696R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14/18/30 kD Observed band size: 15 kD  Sample:A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/44 kD Observed band size: 64 kD  Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-10696R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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