中文名稱 | X連鎖腦特異性BPX蛋白抗體 |
別 名 | BPX; Brain specific gene BPX; Brain specific protein, X linked; Brain-specific protein; MGC26243; Nap1l2; NP1L2_HUMAN; Nucleosome assembly protein 1 like 2; Nucleosome assembly protein 1-like 2; X-linked. |
研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse, Rat, (predicted: Human, Dog, Horse, Rabbit, ) |
產(chǎn)品應(yīng)用 | WB=1:1000-3000 ELISA=1:2000-5000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù)) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 53kDa |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human NHP2L2:141-340/460 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | The protein encoded by this intronless gene is a member of the nucleosome assembly protein (NAP) family. The encoded protein represents a class of tissue-specific factors that interact with chromatin to regulate neuronal cell proliferation. [provided by RefSeq, Jan 2011] Function: Acidic protein which may be involved in interactions with other proteins or DNA. Subcellular Location: Nucleus. Similarity: Belongs to the nucleosome assembly protein (NAP) family. SWISS: Q9ULW6 Gene ID: 4674 Database links: Entrez Gene: 4674 Human Entrez Gene: 17954 Mouse Entrez Gene: 317247 Rat Omim: 300026 Human SwissProt: Q9ULW6 Human SwissProt: P51860 Mouse Unigene: 66180 Human Unigene: 740565 Human Unigene: 388694 Mouse Unigene: 43670 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品圖片 | Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-NHP2L2n (bs-19237R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-NHP2L2n (bs-19237R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (bs-19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (bs-19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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