| 中文名稱 | 精氨酸tRNA連接酶2抗體 |
| 別 名 | arginine tRNA ligase; arginyl tRNA synthetase 2 mitochondrial; Arginyl tRNA synthetase; Arginyl-tRNA synthetase; ArgRS; DALRD2; mitochondrial; PCH6; Probable arginine tRNA ligase; probable arginine tRNA ligase mitochondrial; Probable arginine--tRNA ligase; probable arginyl tRNA synthetase mitochondrial; RARS2; RARSL; SYRM_HUMAN. |
| 研究領域 | 發育生物學 神經生物學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RARS2:221-320/578 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene is an arginyl-tRNA synthetase that is found in the mitochondrial matrix. Defects in this gene are a cause of pontocerebellar hypoplasia type 6 (PCH6). [provided by RefSeq, Oct 2008] Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. SWISS: Q5T160 Gene ID: 57038 Database links: Entrez Gene: 525894 Cow Entrez Gene: 57038 Human Omim: 611524 Human SwissProt: Q0P5H7 Cow SwissProt: Q5T160 Human Unigene: 16559 Cow Unigene: 485910 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Defects in RARS2 are the cause of pontocerebellar hypoplasia type 6 (PCH6) [MIM:611523]; also known as fatal infantile encephalopathy with mitochondrial respiratory chain defects. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. |
| 產品圖片 |  Sample:HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD  Sample:Large intestine (Mouse) Lysate at 40 ug Primary: Anti- RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 62 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RARS2) Polyclonal Antibody, Unconjugated (bs-21159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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