| 來源 | 用途 | 交叉反應性 | 分子量 |
| Rabbit | WB, ICC, IHC, IF | H, M, R, X, C, Cw, Hr, Mk | 42KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | - | 1:100 | 1:100 | 1:100 | - | - | - |
抗體詳細信息如下:
| About this Antibody | |
| Name | α-Smooth Muscle Actin Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody(pAb); Primary antibody |
| Isotype | IgG |
| Purification | Peptide affinity purified |
| About the Immunogen | |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of smooth muscle α-actin. |
| Gene ID | 59 |
| SwissProt | P62736 |
| Synonyms | AAT6; ACTSA; MYMY5; Alpha smooth muscle Actin |
| Category | - |
| Background | Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis. |
包裝清單：
| 產品編號 | 產品名稱 | 包裝 |
| AF0048 | α-Smooth Muscle Actin Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說明書 | 1份 |
保存條件：
α-Smooth Muscle Actin Rabbit Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品僅限于專業人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
使用說明：
請根據抗體的實際用途選擇相應的使用方法。
1. Western檢測：
A. 按照推薦的稀釋比例用提供的Western一抗稀釋液稀釋抗體。
B. 把經過封閉的蛋白膜與稀釋好的一抗4℃緩慢搖動過夜或室溫緩慢搖動2小時，確保稀釋的抗體至少能在搖動的瞬間覆蓋蛋白膜。
C. 回收稀釋的一抗，4℃保存以備下次繼續使用。
D. 按照Western的實驗步驟進行后續的洗滌、二抗孵育、洗滌和檢測等操作。
2. 免疫染色：
可以使用生產的免疫染色一抗稀釋液(P0103)稀釋抗體，使用后注意回收稀釋好的一抗，
3. 其它實驗操作請自行參考適當的protocol進行。
4. 代表性圖片：
 |  |
| Fig. 1. Western blot analysis of α-Smooth muscle actin on different cell lysates using anti-α-Smooth muscle actin antibody at 1/2000 dilution. Positive control: Lane1:Jurkat Lane2: NIH/3T3 Lane 3: Mouse embryonic stem cell Lane 4: Mouse colon. | Fig. 2. Immunocytochemistry staining of α-Smooth muscle actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
 |  |
| Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-α-Smooth muscle actin antibody. Counter stained with hematoxylin. | Fig. 4. Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-α smooth muscle actin antibody. Counter stained with hematoxylin. |
