| 來源 | 用途 | 交叉反應性 | 理論分子量 | 實際分子量 |
| Rabbit | WB, IF, IHC | H, M | 38kDa | 38kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | - | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
抗體詳細信息如下::
| About this Antibody | |
| Name | ANXA1 Rabbit Polyclonal Antibody (KO Validated) |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | HeLa, NIH/3T3 |
| Cellular location | Apical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular side, Lateral cell membrane, Membrane, Nucleus, Peripheral membrane protein, Secreted, cilium, exosome, extracellular space, phagocytic cup, secretory vesicle lumen |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant fusion protein of human ANXA1 (NP_000691.1). |
| Sequence | MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN |
| Gene ID | 301 |
| Swiss Prot | P04083 |
| Synonyms | ANXA1; ANX1; LPC1; annexin A1 |
| Category | Others: Calcium-binding Proteins and Related Molecules |
| Background | The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology. Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis. Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity. Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation. Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function. Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes. Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development. |
包裝清單：
| 產品編號 | 產品名稱 | 包裝 |
| AF5114 | ANXA1 Rabbit Polyclonal Antibody (KO Validated) | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說明書 | 1份 |
保存條件：
ANXA1 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀釋液-20ºC或4ºC保存，一年有效。Western一抗稀釋液優先推薦4ºC保存，長期不使用可以考慮-20ºC保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品僅限于專業人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
使用說明：
請根據抗體的實際用途選擇相應的使用方法。
1. Western檢測：
a. 按照推薦的稀釋比例用Western一抗稀釋液稀釋抗體。
b. 把經過封閉的蛋白膜與稀釋好的一抗4℃緩慢搖動過夜或室溫緩慢搖動2小時，確保稀釋的抗體至少能在搖動的瞬間覆蓋蛋白膜。
c. 回收稀釋的一抗，4℃保存以備下次繼續使用。
d. 按照Western的實驗步驟進行后續的洗滌、二抗孵育、洗滌和檢測等操作。具體操作可以參考如下網頁：
http://www.beyotime.com/western.htm
2. 免疫染色：
可以使用生產的免疫染色一抗稀釋液(P0103)稀釋抗體，使用后注意回收稀釋好的一抗，具體操作可以參考如下網頁：http://www.beyotime.com/support/immunol-staining.htm
3. 其它實驗操作請自行參考適當的protocol進行。
4. 代表性圖片：
 |  |
| Fig. 1. Western blot analysis of extracts of various cell lines, using ANXA1 antibody at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). | Fig. 2. Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s. |
 |  |
| Fig. 3. Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibodyat dilution of 1:100 (40x lens). | Fig. 4. Immunofluorescence analysis of HeLa cells using ANXA1 antibody. Blue: DAPI for nuclear staining. |
