| 來源 | 用途 | 交叉反應性 | 理論分子量 | 實際分子量 |
| Rabbit | WB, IF, IHC | H, M, R | 38/50/75kDa | 51/80kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | - | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
抗體詳細信息如下::
| About this Antibody | |
| Name | TMPO/LAP2 Rabbit Polyclonal Antibody (KO Validated) |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | MCF7, 293T, HepG2, mouse liver, rat liver |
| Cellular location | Chromosome, Nucleus |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant fusion protein of human TMPO (NP_001027454.1). |
| Sequence | MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVPKYVPLADVKSEKTKKGRS |
| Gene ID | 7112 |
| Swiss Prot | P42166/P42167 |
| Synonyms | TMPO; CMD1T; LAP2; LEMD4; PRO0868; TP; thymopoietin |
| Category | Nuclear Receptor Signaling; Chromatin Regulation |
| Background | Lamins and lamina associated proteins (LAP) are the major components of nuclear lamina found between the inner nuclear membrane and the peripheral chromatin. These proteins play important roles in maintaining nuclear structure, chromatin organization, DNA replication, cell cycle regulation, and apoptosis. Lamins are type V intermediate filaments that are further classified into type A and type B lamin proteins. Type A lamins (including lamin A and the smaller lamin C splice variant) are predominately expressed in terminally differentiated cells, whereas type B lamins (lamin B1, lamin B2) are encoded by distinct genes and are expressed constitutively. Cleavage of lamins by caspases occurs during apoptosis as part of the disassembly of the cell. A number of lamina-associated proteins contribute to the nuclear lamina and include the lamin B receptor, LAP1, LAP2, emerin, MAN1, otefin, and YA. Several isoforms of lamina-associated polypeptide 2 (LAP2, also known as thymopoietin or TMPO) have been described, with the α, β, and γ isoforms most abundant in humans. Structurally similar LAP2β (454aa) and LAP2γ (345aa) are type II integral membrane proteins. LAP2α (694aa) has a unique carboxy-terminus that lacks a transmembrane region and results in localization of LAP2α throughout the nucleus where it can associate with lamin A/C. LAP2α is also thought to contribute to the nuclear anchorage of retinoblastoma protein (Rb) and control cell cycle progression. LAP2α is also targeted for cleavage by caspases, which may contribute to changes in chromatin structure during apoptosis. |
包裝清單：
| 產品編號 | 產品名稱 | 包裝 |
| AF5324 | TMPO/LAP2 Rabbit Polyclonal Antibody (KO Validated) | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說明書 | 1份 |
保存條件：
TMPO/LAP2 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀釋液-20ºC或4ºC保存，一年有效。Western一抗稀釋液優先推薦4ºC保存，長期不使用可以考慮-20ºC保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品僅限于專業人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
代表性圖片：
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| Fig. 1. Western blot analysis of extracts of various cell lines, using TMPO antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s. | Fig. 2. Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s. |
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| Fig. 3. Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody at dilution of 1:100 (40x lens). | Fig. 4. Immunofluorescence analysis of U2OS cells using TMPO antibody. |
