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CD90重組兔單克隆抗體

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 中文名稱 CD90重組兔單克隆抗體 

別    名 CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.   
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)   
抗體來源 Rabbit 
克隆類型 Monoclonal 
克 隆 號 2F7 
交叉反應(yīng) Human,  (predicted: Rat, ) 
產(chǎn)品應(yīng)用 WB=1:500-2000 IHC-P=1:50-200 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 
分 子 量 18kDa 
細(xì)胞定位 細(xì)胞膜  
性    狀 Liquid 
濃    度 1mg/ml 
免 疫 原 KLH conjugated synthetic peptide derived from human CD90:  
亞    型 IgG 
純化方法 affinity purified by Protein A 
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 
PubMed PubMed 
產(chǎn)品介紹 Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
 
Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
 
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
 
Tissue Specificity:
Abundant in lymphoid tissues.
 
Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.
 
Similarity:
Contains 1 Ig-like V-type (imm

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD90) Monoclonal Antibody, Unconjugated (bsm-54762R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD90) Monoclonal Antibody, Unconjugated (bsm-54762R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD90) Monoclonal Antibody, Unconjugated (bsm-54762R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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