英文名稱HSP70
中文名稱熱休克蛋白-70單克隆抗體
別 名HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2.
研究領(lǐng)域腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子
抗體來(lái)源Mouse
克隆類型Monoclonal
克 隆 號(hào)5G10
交叉反應(yīng)Human, Mouse, Rat,
產(chǎn)品應(yīng)用WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量70kDa
細(xì)胞定位細(xì)胞漿
性 狀Liquid
濃 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HSP70:
亞 型IgG
純化方法affinity purified by Protein G
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].
Function:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Subunit:
Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3.
Subcellular Location:
Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Tissue Specificity:
HSPA1B is testis-specific.
Similarity:
Belongs to the heat shock protein 70 family.
SWISS:
P0DMV8
Gene ID:
3303
Database links:
Entrez Gene: 281825 Cow
Entrez Gene: 3303 Human
Entrez Gene: 3304 Human
Entrez Gene: 15511 Mouse
Entrez Gene: 193740 Mouse
Entrez Gene: 24472 Rat
Entrez Gene: 294254 Rat
Omim: 140550 Human
Omim: 603012 Human
SwissProt: Q27975 Cow
SwissProt: P0DMV8 Human
SwissProt: P0DMV9 Human
SwissProt: P17879 Mouse
SwissProt: Q61696 Mouse
SwissProt: Q07439 Rat
Unigene: 274402 Human
Unigene: 719966 Human
Unigene: 728810 Human
Unigene: 1950 Rat
Unigene: 228225 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
信號(hào)傳導(dǎo)（Signaling Intermediates）
HSP-70是細(xì)胞受應(yīng)激原刺激后誘導(dǎo)產(chǎn)生的一組應(yīng)激蛋白，與腫瘤發(fā)生、增殖及分化有關(guān)。
環(huán)境和病生理性應(yīng)激原均可導(dǎo)致肌體合成一組應(yīng)急蛋白既熱休克蛋白。許多熱休克蛋白，包括HSP70家族成員，均參與蛋白的變性-復(fù)性、折疊-解折疊、運(yùn)輸-易位、活化-非活化和分泌等過(guò)程。
HSP70與類固醇受體、肌動(dòng)蛋白、P53等蛋白密切相關(guān)。HSP70還參與熱應(yīng)激原、細(xì)胞毒藥物和其他損傷所引起的應(yīng)急反應(yīng)，對(duì)機(jī)體起一定的保護(hù)作用。
| 產(chǎn)品圖片 |  Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-HSP70 (bsm-33048M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-HSP70 (bsm-33048M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (bsm-33048M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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