| Synonyms: | COLO-824COLO-824（人乳腺癌細胞） |
| Background: | established from the pleural fluid of a 52-year-old woman with breast carcinoma in 1991 |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension |
| Growth Mode: | N/A |
| Doubling Time: | ca. 60-80 hours |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+15%FBS We strongly suggest to purchase the complete medium from . |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?) - carries small bisatellited marker |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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