| Synonyms: | HFFF2人類胎兒包皮成纖維細胞 |
| Background: | Derived from a 14-18 week old human foetus. The cells support growth of CMV and HSV. This cell line has a finite life span. |
| Species: | human (Homo sapiens) |
| Tissue: | Foreskin, foetal |
| Disease: | Human Caucasian foetal foreskin fibroblast |
| Gender: | N/A |
| CellType: | Fibroblast |
| Morphology: | N/A |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,12 D16S539: 11,13 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 8,11 vWA: 16,17 Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: |
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