| Cell Line Name: | JOSK-I（人組織細胞淋巴瘤細胞） |
| Cell type: | Histiocytic lymphoma |
| Species: | Homo sapiens，human |
| Description: | Supposedly "established from the peripheral blood of a 72-year-old woman with acute myelomonocytic leukemia (AML FAB M4) in 1983"; however DSMZ DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-I is clearly a derivative of U-937 |
| Morphology: | Single, round to polygonaal cells growing in suspension |
| Mycoplasma: | Negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR + |
| Culture Medium: | DMEM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium from . |
| Cytogenetics: | Human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)<2n>Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/der(7)t(7;18)(q11.23;p11), der(10)t(10;11)(p14;q22.3)/der(10)t(10;11) (p14;q22.3)dup(10)(q25.1), der(11)t(10;11)(p14;q22.3), add(13)(p13), der(17)t(17;18)(p11;q12) - (mar resembles Ph) - carries uncommon 10;11 translocation associated with AML M5 |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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