| I. General information | |
| Synonyms: | WILL-1 |
| Background: | established in 2007 from the bone marrow mononuclear cells of an 82-year-old Japanese man with diffuse large B-cell lymphoma in the leukemic phase |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell lymphoma |
| Gender: | N/A |
| Morphology: | small round or teardrop-shaped cells growing singly or in clumps in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30-50 hours; but 50-70 hours after recovery from liquid nitrogen |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+20%FBS We strongly suggest to purchase the complete medium from |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by species PCR |
| Cytogenetics: | human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8, +9, +11, +13, +der(14), +20, +21; carries t(8;14) effecting IGH/MYC rearrangement confirmed by FISH; non-IGH-MYC rearrangement present in der(6); resembles published karyotype |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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