| I. General information | |
| Synonyms: | U-2940 |
| Background: | established in 1991 from the pleural effusion of a 18-year-old woman with diffuse large B-cell lymphoma (DLBCL) after being diagnosed earlier with Hodgkin lymphoma and treated with chemotherapy; cells were described in the literature to bear microsatellite instability |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell lymphoma |
| Gender: | N/A |
| Morphology: | small round cells growing singly and in clusters in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50 hours |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, cm/cylambda -; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by species PCR |
| Cytogenetics: | human hypodiploid karyotype with 8% polyploidy; 45(43-45)X, -X, del(3)(p14p21), del(6)(q13q15), der(7)t(2;7)(q22;p22), dup(12)(q13q22), der(14)t(X;14)(q12;p11), t(16;16)(p12;p13), del(17)(p13); carries homozygous microdeletion of SOCS1 locus; resembles published karyotype |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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