| Synonyms: | ri-1 |
| Background: | The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration. |
| Species: | human (Homo sapiens) |
| Tissue: | blood |
| Disease: | Human Caucasian B-cell lymphoma |
| Gender: | 48-year-old female |
| Morphology: | Lymphoblastoid |
| Growth Mode: | Suspension |
| Doubling Time: | N/A |
| DNA Profile: | Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18 |
| Subculture Routine: | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present. |
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