| 同用名 | rHuBCA-1/CXCL13; C-X-C motif chemokine 13; BCA1; BLC; SCYB13 ; 重組人源 CXC 趨化因子13 (CXCL13) |
|---|---|
| 種屬 | Human |
| 表達(dá)系統(tǒng) | E. coli |
| 蛋白編號(hào) | O43927 (V23-P109) |
| 基因 ID | 10563 |
| 分子量 | Approximately 10.3 kDa |
| 氨基酸序列 | VLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP |
| 生物活性 | Full biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/mL. |
| 性狀 | Lyophilized powder. |
| 組分 | Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 100 mM NaCl. |
| 內(nèi)毒素含量 | <1 EU/μg, determined by LAL method. |
| 復(fù)溶方法 | It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water or aqueous buffer containing 0.1% BSA. |
| 保存條件 & 期限 | Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
| 運(yùn)輸條件 | Room temperature in continental US; may vary elsewhere. |
| 描述 | Human BCA-1/CXCL13 is identified as an efficacious and selective attractant for B lymphocytes, also acts as a B cell homing chemokine. Human BCA-1 competes with radiolabeled interferon gamma (IFN-γ) inducible protein 10 (IP-10) for binding to the human CXCR3 receptor. Furthermore, human BCA-1 is an efficacious attractant for human CXCR3 transfected cells; BCA-1-induced chemotaxis is inhibited by a monoclonal antibody against human CXCR3. Indeed, BCA-1 attenuates the calcium flux induced by IP-10. In addition, human BCA-1 is an agonist in stimulating GTPγS binding[1]. |
