Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- OSM-dependent endothelial-like cell line, LO
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Onitsuka, I., et al., 2010. Gastroenterology, 138:1525-1535.
- Doyonnas, R., et al., 2005. Blood, 105:4170-4178.
- Minegishi, N., et al., 2003. Blood, 102:896-905.
- Minehata, K., et al., 2002. Blood, 99:2360-2368.
- Doyonnas, R., et al., 2001. J. Exp. Med., 194:13-28.
- Hara, T., et al., 1999. Immunity, 11:567-578.
- RRID
- AB_2922457 (BioLegend Cat. No. 107903)
Antigen Details
- Structure
- Single-pass type I membrane protein, sialoglycoprotein
- Distribution
-
Apical cell membrane, membrane raft
- Function
- Cell adhesion, cell migration
- Interaction
- Forms a complex with EZR and SLC9A3R2
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
- Antigen References
-
- Refaeli I, et al. 2020. Scientific Reports 10:9419
- Economou C, et al. 2004. J Cell Sci (2004) 117 (15): 3281–94.
- Cait J, et al. 2019. Proc Natl Acad Sci. 116 (10) 4518-4527
- Doyonnas R, et al. 2005. Blood. 105(11):4170-8
- Zhang H, et al. 2014. Stem Cells. 32(1):191-203
- Gene ID
- 27205 View all products for this Gene ID
- UniProt
- View information about Podocalyxin on UniProt.org
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