Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MIF with a His tag
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this protein is quality control tested by ELISA assay. A concentration of 0.125 ?g/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.
- Application Notes
ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody.
Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1.The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (contact our custom solutions team).
Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX? ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.- Application References
(PubMed link indicates BioLegend citation) -
- Zhou H, et al. 2009. PLoS ONE. 4:e6087. (FA, Neut)
- RRID
- AB_2563135 (BioLegend Cat. No. 525503) AB_2563136 (BioLegend Cat. No. 525504)
Antigen Details
- Structure
- Predominantly a homotrimer
- Distribution
-
Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells
- Function
- Pro-inflammatory cytokine, regulates macrophage function, involved in cancer development, tautomerase activity
- Interaction
- Monocytes and macrophages, mesenchymal cells, B-cells
- Ligand/Receptor
- CD74, CXCR4, CXCR2, CXCR7
- Cell Type
- Endothelial cells, Eosinophils, Epithelial cells, Macrophages, T cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Zhou H, et al. 2009. PLoS ONE. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737. - Gene ID
- 4282 View all products for this Gene ID
- UniProt
- View information about MIF on UniProt.org
